Composite

Part:BBa_K1111000

Designed by: Luisa Spisak   Group: iGEM13_EPF_Lausanne   (2013-09-18)


Hya promoter_GFP

This promoter was assembled together with superfolded GFP. The hya promoter itself is sensitive to pH, and it is induced when the pH is around 5.5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1249


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Categories
Parameters
None